mrtour at mrtour at
Tue May 14 10:31:32 EST 1996

In article <bilha001-1205961553460001 at>,
bilha001 at (Julaine) wrote:

>    I plan to do immunohistochemistry looking at a nuclear antigen on two
> color FACS sorted cells and cytospun on to a slide. My primary antibody
> for the nuclear antigen is of the same mouse isotype as the primary
> antibody used to sort with the FACS. I predict a cross reaction problem
> when I come back with the secondary Rabbit anti-mouse labled antibody. Any
> quick advice on how to eliminate this background will be appreciated. 
>      A reply by email is fine bilha001 at

protease treatment?



or Michelle

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