IUBio

postdoctoral position wanted

Zhugong Liu tclab at IRIS.BJMU.EDU.CN
Thu Nov 7 09:05:22 EST 1996


Dear Sir:

I am writing for the possibility of doing postdoctoral research work
in one laboratory.

As outlined in my CV, my research work is mainly focus on T cell 
and Chemokines, and the associated technology. I hope to get a 
postdoctoral position in the related research areas. I am sure 
that my experience, techniques and hard working will be very 
helpful for your research work. 

Currently, I am in the process of doing my Ph.D., and I am scheduled
to finish my Ph.D. program in May of 1997 here at Beijing Medical
University. I hope to do further research in the related areas, 
starting from July, 1997.

Enclosed please find my C.V. for your consideration. Please feel free
to contact me if you require any further information.

I would appreciate it greatly if you can take me into
your consideration.


Sincerely yours,


Zhu-Gong Liu
Dept. of Immunology
Beijing Medical University
Beijing, 100083
CHINA
============================================================
                          CURRICULUN VITAE


NAME:                  Zhugong Liu

ADDRESS:               T Cell Research Lab
                       Department of Immunology
                       Beijing Medical University
                       Beijing, 100083
                       P. R. China

Telephone:             86-10-6209-1155
Fax :                  86-10-6209-1436
Email  address:        tclab at mail.bjmu.edu.cn

SEX:                   Male

HEALTH:                Excellent

DATE OF BIRTH:         October 16, 1969

NATIONALITY:           Chinese

EDUCATION:

1987-1992             undergraduate student (Bachelor), Department of 
                      Basic Medicine, Beijing Medical
                      University, Beijing, China.

1992-present          graduate student (Ph.D.), Department of 
                      immunology, Beijing Medical University

RESEARCH EXPERIENCES:

1991-1992       establishment of mouse thymic stromal cell (MTSC)
                lines; Cell cloning of MTSC lines; involved in
                establishment of human tumor model in nude mouse.
                evaluation of antitumor activity of IL-4 against
                human tumors.

1992-1993       involved in a research project supported by
                CMB (Chinese Medical Board, USA): 
                Characterization of established MTSC lines and
                clones, including the detection of keratin, vimentin,
                S-100, Mac-1, Ia, Thy-1 and several markers for
                dendritic cells; involved in detection of the
                cytokines (IL-1, IL-2, IL-3, IL-6, IFN) secreted by
                MTSC cells; Preparation of monoclonal antibodies
                against thymic stromal cells; The types and subtypes
                of MTSC cells identified by these McAbs.

1993-1994       as a main cooperator in China, involved in a project 
                supported by European Committee ( in cooperation
                with Dr. Van Damme). Detection of chemotactic factors
                secreted by MTSC lines; Establishment of a method
                that can be used to analysis the phenotype and number
                of migrated lymphocytes; Purification of chemokines
                from MTSC culture supernatant. After RP-HPLC, a
                single protein bond of 30 KDa was seen  in
                silver-stained SDS-PAGE.

1994-1995       amino acid sequence analysis of the 30 KDa chemokine; 
                Analysis of chemotactic activity of this chemokine on
                bone marrow cells and the phenotype of migrated
                BM cells; Detection of chemotactic activity of
                this chemokines on different subsets of thymocyte.

1995-Present    Analysis of TCR gene rearrangement, phenotypic
                and functional maturation of thymocyte induced
                by this chemokine, including adhesion molecule
                expression on thymocyte; Cloning of chemokine
                gene from MTSC using RACE-PCR; Another
                chemokine of 7KDa was found; Purification of 
                this chemokine to homogeneity.

EXPERIMENT SKILLS

1.  Immunology: Detection of cytokines (such as IL-1, IL-2, IL-4,
                IL-6, GM-CSF, chemokines, etc.) by bioassay 
                and ELISA; Phenotype and function  analysis of
                different subsets of lymphocyte; Indirect
                immunofluorescence and immunohistochemistry
                techniques; Isolation of lymphocyte subsets
                with high purity using Panning, complement
                mediated cytotoxi- city and Immunobeats
                techniques; Use of lab animals; Preparation of
                McAbs. Labeling of McAb with FITC or PE.

2. Cellular Biology: Cell culture; Cell cloning; Establishment of
                     cell lines; FACS analysis; Histochemistry;
                     3H-TdR uptake assay and MTT assay;
                     Electrophoresis techniques.

3. Molecular Biology: Purification of proteins, including Affinity
                      chromatography, Ion-exchange chromatography,
                      Gel filtration, FPLC and HPLC; Protein
                      sequencing; SDS-PAGE; Silver stain; Agrose
                      electrophoresis; DNA preparation; mRNA
                      isolation; PCR; RT-PCR; RACE-PCR; Recombinant
                      DNA techniques.

AWARDS

1991            Special Grade Award for students of Beijing Medical
                University 

1992            Award for excellent graduation thesis of
                Beijing Medical University 

1992            Named as Outstanding Graduate of Beijing Medical 
                University 

1994            Guanghua Award for graduate students of Beijing 
                Medical University

1996            (1)Guanghua Award for graduate students of Beijing 
                   Medical University
                (2)FIMSA (The Federation of Immunological
                   Societies of Asia-Oceania) First Congress 
                   Post-Graduate Student Travel Award (the only
                   one in China)


PUBLICATIONS:

1. Sun SZ, Chen WF, Liu ZG, and Pang XW: Establishment and 
    identification of eight mouse thymic stromal cell lines. J Beijing
    Med. Univ. 25: 237, 1993.
2. Pang XW, Chen WF, Shu SZ, Liu ZG, and Zhang PZ: Spontaneous
    secretion of IFN by established mouse thymic stromal cell lines.
    Chin J Microbiol. & Immunol. 13:205, 1993.
3. Yu Q, Chen WF, Pang XW, Qian XP, and Liu ZG: The biological
    activity of interleukin 7 produced by nine established murine
    thymus stromal cell lines. Chin J Microbiol & Immunol. 13:291,
    1993.
4. Pang XW, Qian XP, Liu ZG, and Chen WF: The levels of interleukin 1
    and interleukin 6 production by established eight mouse thymic
    stromal cell lines. Chin J Immunol.    9:328, 1993.  
5. Liu ZG, Chen WF, Cao LX, Pang XW, and Yu Q: Cytokine production by
    MTSC4 cells, an established mouse thymic dendritic cell line. Acta
    Biol. Experiment Sinica. 27: 91, 1994.
6. Nong GH., Chen WF, and Liu ZG: The types and subtypes of
    established mouse thymic stromal cell lines identified by
    monoclonal antibodies. Chin J Microbiol & Immunol. 14:149, 1994.
7. Liu ZG, Miao YL, Cao LX, and Chen WF: Mouse  thymic epithelial cell
    line MTEC1 produced IL-8-like chemokines. J Beijing Med. Univ.
    27:67,1995.
8. LiuQ, ZhangPX, Liu ZG, Wu JS, and Chen WF: Establishment and
    characterization of mouse bone marrow stromal cell line (BMSC).
    Chin Sci Bull. 41:744, 1996.
9. Liu ZG, Fang L and Chen WF: Analysis of chemokines produced by
    mouse thymic stromal cell lines. 1995. Acta Biol. Exp. Sinica.
    29:25, 1996.
10.Liu Q, Zhang PX, Liu ZG, Qian XP, Pang XW and Chen WF: Multiple
     types of cytokines comtitutively produced by mouse bone marrow
     stromal cell line BMSC1. Chin J Microbiol & Immunol. 16:113,
     1996.
11.Liu ZG, Chen WF: The development of research on chemokine
     superfamily.(review) Life Sciences. 8:1, 1996.
12.Zhang PX, Zhang GR, Xiao SY, Liu ZG and Chen WF: Study on the
     effects of mouse thymic epithelial cell line MTEC1 after
     intrasplenic injection of nude mice. Chin J Microbiol & Immunol.
     16:171, 1996.
13.Liu Q, Cao LS, Liu ZG, Wu JS and Chen WF: Establishment and
     characterization of mouse fetal liver stromal cell lines. 1995.
     Chin J Microbiol & Immunol. (Accepted)
14.Liu ZG, Haelens A, Wuyts A, Struyf S, Pang XW, Proost P, Chen WF,
     and Van Damme J.: Isolation of a lymphocyte chemotactic factor
     produced by the established murine thymic epithelial cell line
     MTEC1: Identification as a 30 Kda glycosylated form of  MCP-1.
     1996. Eur. Cytokine Network. 7:381,1996.
15.Liu ZG and Chen WF: The mechanism of T cell migration.(review) Life
     Scinece.  (Submitted)
16.Liu ZG and Chen WF: Interleukin 16. (review) Life Science.
     (Submitted)
17. Liu Q, Liu ZG and Chen WF: Characterization and cytokines 
      secretion of a mouse fetal liver stromal cell line. Acta Anatomy. 
       (Submitted)
18.  Pei LH, Liu ZG and Chen WF: Establishment, characterization 
        and cytokines secretion of mouse thymic dendritic cell clones. 
         Chin J Microbiol & Immunol. (Submitted)



REFERENCES:

1. Dr. Wei-Feng Chen, M.D., Ph.D.
            Professor of Immunology
            Member, Chinese Academy of Sciences
            Council number of IUIS (International Union of 
                    Immunological Societies)

 Address:  Department of Immunology
           Beijing Medical University
           Beijing, 100083
           P. R China
 Telephone(FAX): 86-10-6209-1436
 Email: wfchen at public.bta.net.cn

2. Dr. Zhen-Zhou Long, M.D. 
              Professor of Immunology
              Dean of Department of Immunology
 Address: Department of Immunology
          Beijing Medical University
          Beijing, 100083
          P. R. China
 Telephone: 86-10-6209-1156

3. Dr. Hong-Ti Jia, M.D.
           Professor of Biochemistry
           Dean of Department of Molecular Biology & Biochemistry
 Address: Department of Molecular Biology & Biochemistry
          Beijing Medical University
          Beijing, 100083
          P. R. China
 Telephone: 86-10-6209-1434



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