Dear Sir:
I am writing for the possibility of doing postdoctoral research work
in one laboratory.
As outlined in my CV, my research work is mainly focus on T cell
and Chemokines, and the associated technology. I hope to get a
postdoctoral position in the related research areas. I am sure
that my experience, techniques and hard working will be very
helpful for your research work.
Currently, I am in the process of doing my Ph.D., and I am scheduled
to finish my Ph.D. program in May of 1997 here at Beijing Medical
University. I hope to do further research in the related areas,
starting from July, 1997.
Enclosed please find my C.V. for your consideration. Please feel free
to contact me if you require any further information.
I would appreciate it greatly if you can take me into
your consideration.
Sincerely yours,
Zhu-Gong Liu
Dept. of Immunology
Beijing Medical University
Beijing, 100083
CHINA
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CURRICULUN VITAE
NAME: Zhugong Liu
ADDRESS: T Cell Research Lab
Department of Immunology
Beijing Medical University
Beijing, 100083
P. R. China
Telephone: 86-10-6209-1155
Fax : 86-10-6209-1436
Email address: tclab at mail.bjmu.edu.cn
SEX: Male
HEALTH: Excellent
DATE OF BIRTH: October 16, 1969
NATIONALITY: Chinese
EDUCATION:
1987-1992 undergraduate student (Bachelor), Department of
Basic Medicine, Beijing Medical
University, Beijing, China.
1992-present graduate student (Ph.D.), Department of
immunology, Beijing Medical University
RESEARCH EXPERIENCES:
1991-1992 establishment of mouse thymic stromal cell (MTSC)
lines; Cell cloning of MTSC lines; involved in
establishment of human tumor model in nude mouse.
evaluation of antitumor activity of IL-4 against
human tumors.
1992-1993 involved in a research project supported by
CMB (Chinese Medical Board, USA):
Characterization of established MTSC lines and
clones, including the detection of keratin, vimentin,
S-100, Mac-1, Ia, Thy-1 and several markers for
dendritic cells; involved in detection of the
cytokines (IL-1, IL-2, IL-3, IL-6, IFN) secreted by
MTSC cells; Preparation of monoclonal antibodies
against thymic stromal cells; The types and subtypes
of MTSC cells identified by these McAbs.
1993-1994 as a main cooperator in China, involved in a project
supported by European Committee ( in cooperation
with Dr. Van Damme). Detection of chemotactic factors
secreted by MTSC lines; Establishment of a method
that can be used to analysis the phenotype and number
of migrated lymphocytes; Purification of chemokines
from MTSC culture supernatant. After RP-HPLC, a
single protein bond of 30 KDa was seen in
silver-stained SDS-PAGE.
1994-1995 amino acid sequence analysis of the 30 KDa chemokine;
Analysis of chemotactic activity of this chemokine on
bone marrow cells and the phenotype of migrated
BM cells; Detection of chemotactic activity of
this chemokines on different subsets of thymocyte.
1995-Present Analysis of TCR gene rearrangement, phenotypic
and functional maturation of thymocyte induced
by this chemokine, including adhesion molecule
expression on thymocyte; Cloning of chemokine
gene from MTSC using RACE-PCR; Another
chemokine of 7KDa was found; Purification of
this chemokine to homogeneity.
EXPERIMENT SKILLS
1. Immunology: Detection of cytokines (such as IL-1, IL-2, IL-4,
IL-6, GM-CSF, chemokines, etc.) by bioassay
and ELISA; Phenotype and function analysis of
different subsets of lymphocyte; Indirect
immunofluorescence and immunohistochemistry
techniques; Isolation of lymphocyte subsets
with high purity using Panning, complement
mediated cytotoxi- city and Immunobeats
techniques; Use of lab animals; Preparation of
McAbs. Labeling of McAb with FITC or PE.
2. Cellular Biology: Cell culture; Cell cloning; Establishment of
cell lines; FACS analysis; Histochemistry;
3H-TdR uptake assay and MTT assay;
Electrophoresis techniques.
3. Molecular Biology: Purification of proteins, including Affinity
chromatography, Ion-exchange chromatography,
Gel filtration, FPLC and HPLC; Protein
sequencing; SDS-PAGE; Silver stain; Agrose
electrophoresis; DNA preparation; mRNA
isolation; PCR; RT-PCR; RACE-PCR; Recombinant
DNA techniques.
AWARDS
1991 Special Grade Award for students of Beijing Medical
University
1992 Award for excellent graduation thesis of
Beijing Medical University
1992 Named as Outstanding Graduate of Beijing Medical
University
1994 Guanghua Award for graduate students of Beijing
Medical University
1996 (1)Guanghua Award for graduate students of Beijing
Medical University
(2)FIMSA (The Federation of Immunological
Societies of Asia-Oceania) First Congress
Post-Graduate Student Travel Award (the only
one in China)
PUBLICATIONS:
1. Sun SZ, Chen WF, Liu ZG, and Pang XW: Establishment and
identification of eight mouse thymic stromal cell lines. J Beijing
Med. Univ. 25: 237, 1993.
2. Pang XW, Chen WF, Shu SZ, Liu ZG, and Zhang PZ: Spontaneous
secretion of IFN by established mouse thymic stromal cell lines.
Chin J Microbiol. & Immunol. 13:205, 1993.
3. Yu Q, Chen WF, Pang XW, Qian XP, and Liu ZG: The biological
activity of interleukin 7 produced by nine established murine
thymus stromal cell lines. Chin J Microbiol & Immunol. 13:291,
1993.
4. Pang XW, Qian XP, Liu ZG, and Chen WF: The levels of interleukin 1
and interleukin 6 production by established eight mouse thymic
stromal cell lines. Chin J Immunol. 9:328, 1993.
5. Liu ZG, Chen WF, Cao LX, Pang XW, and Yu Q: Cytokine production by
MTSC4 cells, an established mouse thymic dendritic cell line. Acta
Biol. Experiment Sinica. 27: 91, 1994.
6. Nong GH., Chen WF, and Liu ZG: The types and subtypes of
established mouse thymic stromal cell lines identified by
monoclonal antibodies. Chin J Microbiol & Immunol. 14:149, 1994.
7. Liu ZG, Miao YL, Cao LX, and Chen WF: Mouse thymic epithelial cell
line MTEC1 produced IL-8-like chemokines. J Beijing Med. Univ.
27:67,1995.
8. LiuQ, ZhangPX, Liu ZG, Wu JS, and Chen WF: Establishment and
characterization of mouse bone marrow stromal cell line (BMSC).
Chin Sci Bull. 41:744, 1996.
9. Liu ZG, Fang L and Chen WF: Analysis of chemokines produced by
mouse thymic stromal cell lines. 1995. Acta Biol. Exp. Sinica.
29:25, 1996.
10.Liu Q, Zhang PX, Liu ZG, Qian XP, Pang XW and Chen WF: Multiple
types of cytokines comtitutively produced by mouse bone marrow
stromal cell line BMSC1. Chin J Microbiol & Immunol. 16:113,
1996.
11.Liu ZG, Chen WF: The development of research on chemokine
superfamily.(review) Life Sciences. 8:1, 1996.
12.Zhang PX, Zhang GR, Xiao SY, Liu ZG and Chen WF: Study on the
effects of mouse thymic epithelial cell line MTEC1 after
intrasplenic injection of nude mice. Chin J Microbiol & Immunol.
16:171, 1996.
13.Liu Q, Cao LS, Liu ZG, Wu JS and Chen WF: Establishment and
characterization of mouse fetal liver stromal cell lines. 1995.
Chin J Microbiol & Immunol. (Accepted)
14.Liu ZG, Haelens A, Wuyts A, Struyf S, Pang XW, Proost P, Chen WF,
and Van Damme J.: Isolation of a lymphocyte chemotactic factor
produced by the established murine thymic epithelial cell line
MTEC1: Identification as a 30 Kda glycosylated form of MCP-1.
1996. Eur. Cytokine Network. 7:381,1996.
15.Liu ZG and Chen WF: The mechanism of T cell migration.(review) Life
Scinece. (Submitted)
16.Liu ZG and Chen WF: Interleukin 16. (review) Life Science.
(Submitted)
17. Liu Q, Liu ZG and Chen WF: Characterization and cytokines
secretion of a mouse fetal liver stromal cell line. Acta Anatomy.
(Submitted)
18. Pei LH, Liu ZG and Chen WF: Establishment, characterization
and cytokines secretion of mouse thymic dendritic cell clones.
Chin J Microbiol & Immunol. (Submitted)
REFERENCES:
1. Dr. Wei-Feng Chen, M.D., Ph.D.
Professor of Immunology
Member, Chinese Academy of Sciences
Council number of IUIS (International Union of
Immunological Societies)
Address: Department of Immunology
Beijing Medical University
Beijing, 100083
P. R China
Telephone(FAX): 86-10-6209-1436
Email: wfchen at public.bta.net.cn
2. Dr. Zhen-Zhou Long, M.D.
Professor of Immunology
Dean of Department of Immunology
Address: Department of Immunology
Beijing Medical University
Beijing, 100083
P. R. China
Telephone: 86-10-6209-1156
3. Dr. Hong-Ti Jia, M.D.
Professor of Biochemistry
Dean of Department of Molecular Biology & Biochemistry
Address: Department of Molecular Biology & Biochemistry
Beijing Medical University
Beijing, 100083
P. R. China
Telephone: 86-10-6209-1434
