Fixing tissues

[Anthony Pang]

I read somewhere that using formaldhyde in fixing tissues will
cross-link tissues in the sections. This may cause a problem in receptor
detection. Is this true? I am looking for the formula for Bouin-Hollande
10% sublimate ( P. Brandtzaeg: Tissue preparation methods for
immunocytochemistry IN "Techniques in Immunocytochemistry Vol 1, edited
by G.R. Bullock and P. Petrusz, 1982"- I don't have this volume)

Please send formula to : apang at forestry.ca