Help with HIV elisa

snowtrek at snowtrek at
Fri Nov 8 00:08:48 EST 1996

* Did you dialyze the lysate to get rid of the detergent?
* What kind of plates are you using?
* Is your "anti-HIV antibody" polyclonal? Monoclonal? Epitope of interest?
Point being, you could coat the plate with an antibody (that sees an
epitope other than the one(s) recognized by your anti-HIV antibody) in
order to capture the molecule of interest from the lysate.

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