ELISA

[Robert Somogyi]

On 15 Nov 1996, sherry weppler wrote:

> What other enzymes can be used, where do they come from, what 
> is their substrate, and what is the detection colour?
> 
having done many myself....i tend to go towards HRP congugated secondary 
antibodies (horse radish peroxidase)....

this can be bought from santa cruz biotechnlogy (fairly cheap)

the substrate is TMB (available from sigma)

read the results at O.D. 450 nm

(heck...im doing this today in fact)

hope it helps

robert