ELISA

sherry weppler sweppler at mustang.uwo.ca
Thu Nov 14 20:51:34 EST 1996


I am an undergraduate student taking an introductory immunology 
course. I have just finished a series of lab sessions in which 
we immunized mice with ovalbumin and an alum adjuvant, bled 
them, and preformed ELISA to calculate the concentration of 
ovalbumin antibodies in the plasma. It has now come time to 
write up the lab report, and we are to include in our 
discussion some information about capture ELISA and sandwich 
ELISA. The enzyme we used in the experiment was alkaline 
phosphatase with para-nitrophenylphosphate as the substrate.  
What other enzymes can be used, where do they come from, what 
is their substrate, and what is the detection colour?

I would really appreciate some information as I don't have much 
time to spend in the library. 
Thanks




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