fab fragments from Rat IgG2a

rjjensen at inav.net rjjensen at inav.net
Thu Nov 21 20:50:10 EST 1996

I am wanting to make fab fragments from a rat IgG2a that was purified
from a serum free hybridoma supe. Pierce Chemical sells immobilized
pappain, but I am afraid the fab fragment after digestion will be
difficult to purify via Protein G. If any of you could send me a
reference for making fabs from rat IgG2a I would greatly appreciate your
help. The only reference I have is from 1983 (before the advent of
Protein G) My E-mail address is rjjensen at inav.net  Thanks for your help.

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