Fixing tissues

Ayrton Queiroga ayrtonq at icanect.net
Fri Nov 22 15:58:26 EST 1996


Christine Pechoux wrote:
> 
> In article (Dans l'article) <32813601.5579 at forestry.ca>, Anthony, Pang:,
> apang at forestry.ca wrote (écrivait) :
> 
> > I read somewhere that using formaldhyde in fixing tissues will
> > cross-link tissues in the sections. This may cause a problem in receptor
> > detection. Is this true? I am looking for the formula for Bouin-Hollande
> > 10% sublimate ( P. Brandtzaeg: Tissue preparation methods for
> > immunocytochemistry IN "Techniques in Immunocytochemistry Vol 1, edited
> > by G.R. Bullock and P. Petrusz, 1982"- I don't have this volume)
> >
> > Please send formula to : apang at forestry.ca
> 
> Hi,
> I some experiences in immunochemistry, and tissues fixation, so
> commercialy formaldhyde is not the best fixative existed. And
> Bouin-Hollande is really bad, and if you can avoid to use it !
> So paraformaldehyde fixative is is the most widely used fixative, either
> with or without the addition of glutaraldehyde.
> The paraformaldehyde concentration is between 1 to 4%, and high
> glutaraldahyde concentration (>0,5%) increased structural preservation,
> but also decreased the signal.
> So try both fixatives alone or mixed and goog luck
> Christine

Why not use frozen secctions?
-- 
MZ



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