?Coating efficiency of an ELISA plate?

Zafer Altin zafer at abconcepts.com
Fri Nov 22 17:12:02 EST 1996


Biotinylation efficiency, in like any other labeling method will depend
on the availability of reactive groups. Since he is looking into a set
of largely similar proteins, one may expect that he will get similar
efficiencies. Biotin/Protein molar ratios will be a good indication
about the state of biotinylated collagens. I will be content with 
27 vs 30 b/p but would be concerned (2 vs 25 b/p) before proceeding with
the comparative study.  We have utilized above procedure for peptide
binding efficiencies and worked well.
  
I agree that a more precise determination of antigen binding efficiency
will require use of radioactive tracer but gentleman does not wish to do
that.

Best Regards,

Zafer



More information about the Immuno mailing list