In article <52rc1k$1omo at news.doit.wisc.edu>,
Nicole <nglowack at students.wisc.edu> wrote:
>To gently remove macrophages and other adherent cells from culture dishes,
>try trypsin-versene mixture. It should be in the Sigma Cell Culture
>catalog. Good luck!
>>Nicole
>
You can also try using 1mM EDTA in PBS. Since most of the adhesion molecules
require divalent cations, chelation by EDTA allows removal of cells without
any proteolysis.
Ken
--
Ken Frauwirth (MiSTie #33025) _ _
frauwirt at mendel.berkeley.edu |_) * |/ (_ |\ |
http://www.ocf.berkeley.edu/~frauwirt/ |_) | () |\ (_ | \|
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs
