macrophages/FACS analysis

[Ken Frauwirth BioKen]

In article <52rc1k$1omo at news.doit.wisc.edu>,
Nicole  <nglowack at students.wisc.edu> wrote:
>To gently remove macrophages and other adherent cells from culture dishes, 
>try trypsin-versene mixture.  It should be in the Sigma Cell Culture 
>catalog.  Good luck!
>
>Nicole 
>

You can also try using 1mM EDTA in PBS.  Since most of the adhesion molecules
require divalent cations, chelation by EDTA allows removal of cells without
any proteolysis.  


Ken

-- 
Ken Frauwirth (MiSTie #33025)                         _           _
frauwirt at mendel.berkeley.edu                         |_) *    |/ (_ |\ |
http://www.ocf.berkeley.edu/~frauwirt/               |_) | () |\ (_ | \|  
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs