macrophages/FACS analysis

Ken Frauwirth BioKen frauwirt at bacillus.Berkeley.EDU
Tue Oct 1 12:12:10 EST 1996

In article <52rc1k$1omo at>,
Nicole  <nglowack at> wrote:
>To gently remove macrophages and other adherent cells from culture dishes, 
>try trypsin-versene mixture.  It should be in the Sigma Cell Culture 
>catalog.  Good luck!

You can also try using 1mM EDTA in PBS.  Since most of the adhesion molecules
require divalent cations, chelation by EDTA allows removal of cells without
any proteolysis.  


Ken Frauwirth (MiSTie #33025)                         _           _
frauwirt at                         |_) *    |/ (_ |\ |               |_) | () |\ (_ | \|  
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs

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