macrophages/FACS analysis

Now at there.edu Now at there.edu
Wed Oct 2 12:54:48 EST 1996


Try using an EDTA-PBS solution.  Then gently blow off the cells with a
pipet.  Generally, macrophages, lymphocytes, etc. will tend to shed their
receptors even under this condition when released from tissue culture
plastic.  Unfortunately this is the nature of these cells.


In article <32510893.2B64 at nimr.mrc.ac.uk>, anybody
<anybody at nimr.mrc.ac.uk> wrote:

> I am looking at apoptosis in infected macrophages, this is an 'in vitro'
> study.  The problem is this : How do I remove them from the culture
> vessel without losing the cell surfaces markers? I will require to know
> the cell type of the apoptotic cells.
> 
> Best
> 
> b-butler at nimr.mrc.ac.uk



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