using antigens in gels

Zafer Altin, Ph.D. zafer at
Fri Oct 4 04:53:43 EST 1996

You can cut and freeze the band of gel corresponding to your antigen in 
liquid nitrogen and crush frozen gel bits with a mortar and pestle
(a small one,  I suggest a pyrex one). Then you can scoop it up with a 
spatula and mix with your adjuvant.  If you have to add additional liq 
nitrogen while making into a fine powder, add small quantities of liq 
nitrogen so that your prep does not end up on the bench. Good luck!

Zafer Altin, Ph.D.
Antibody Concepts
So. San Francisco, CA  USA

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