SDS-PAGE of small proteins/peptides

Frode Vartdal frode.vartdal at rh.uio.no
Fri Oct 4 03:57:18 EST 1996


I am working on separation of low molecular weight (LMW) proteins/peptides (MW 2 -8 kDa) in SDS-polyacrylamide 
electrophoresis. The gels are fixed in a solution containing 40% methanol, 10% acetic acid 
and 50% aqua. dest. My problem is that I loose most of the LMW-material while the gels are fixed and 
stained (silver). I'd be interested to get information about fixation procedures which also fix small proteins
in the gel. 

Thanks.


Frode



Frode Vartdal
Institute of Transplantation Immunology
Rikshospitalet
University of Oslo
0027 Oslo
Norway

Fax: +47 22 20 36 93
Tel: +47 22 86 85 65/ +47 22 86 85 55 



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