Treating TEM-cuts with whole-mount gold AB

Jens Krieger krieger at
Mon Oct 7 09:35:09 EST 1996

I've a problem with my ultrathin cuts for AB-staining treating them with
IgG-whole gold mount AB from Sigma. Before I use the AB I treated them
with 1% BSA in PBS+TWEEN 20 for about half an hour. Then I use my first AB
in PBS+TWEEN for three hours at roomtemperatur. Washing three times in
PBS+TWEEN, incubating with the gold in PBS+TWEEN for about two hours at
RT, drying is a very high background on my grids. Like
the BSA falls out or anything else. I can't explain...
Does anyone know why there is such a high background? Or does anyone has a
hint how a can improvet the immunolabeling of my cuts?
Thanks a lot in advance,

|  Jens Krieger		"Solange die Alpen noch stehen, |
|  				wird es keine		|
|  Tel.:+49.351.463.5465  	   Gerechtigkeit geben."|
|							|			
|  krieger at	H.Achternbusch	|
\  krieger at			/

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