Purification of IgM & C4 from human serum

Jeffrey Smoot jsmoot at cas.org
Tue Oct 8 11:36:44 EST 1996


Library wrote:
> 
> Hello-
> I am looking for any ideas on how to extract (not necessarily purify)
> either IgM or C4 (Complement 4) from raw human serum.  We are exploring
> ways of elevating either protein in calibrator products and are looking
> for any helpful suggestions.  Thanks.
> -Janene Lenard
>  Protein Chemist

Janene, perhaps these citations will be useful.

-- 
Jeffrey Smoot
Chemical Abstract Services            Phone    614-447-3600 X3198
P.O. Box 3012                         E-mail   mailto: jsmoot at cas.org
Columbus, OH 43210                    WWW      http://www.cas.org
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TI   Purification of human immunoglobulin M by affinity chromatography on
     protamine-Sepharose
AU   Wichman, Anders; Borg, Hakan
CS   Res. Dep. Biochem., AB Kabi, Stockholm, Swed.
SO   Biochim. Biophys. Acta (1977), 490(2), 363-9


TI   Separation of different forms of the fourth component of human
     complement by fast protein liquid chromatography
AU   Hessing, Martin; Paardekooper, Jolanda; Hack, C. Erik
CS   Cent. Lab. Neth. Red Cross Transfus. Serv., Amsterdam, Neth.
SO   J. Immunol. Methods (1993), 157(1-2), 39-48


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