conA binding to antibodies

Simon Easterbrook-Smith sbe at biochem.usyd.edu.au
Wed Oct 9 07:09:50 EST 1996


Keld Sorensen wrote:
> 
> Con A will only bind to anything in the presence
> of Mg or Mn, so simply use buffers without that
> and no binding will occur....
> 
> Keld.

I have joined this thread late, so sorry if I have missed something.

As far as I know, while Con A is a metalloprotein (needing bound Mg/Mn)
as long as these are not stripped from it (with high concns of EDTA, low
pH treatment, whatever) it will bind to target proteins fine in the
absence of exogeneous Mg/Mn.

Simon



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