immunoblot-help needed!

ManiUSA at ManiUSA at
Sun Oct 13 22:16:58 EST 1996

     I have a persistent problem with my immunoprecipitation experiments. My
film  is full of small spots after I perform the chemiluminescence step, even
though the bands are clearly visible. I spin down the antibodies before use
them. I block the PVDF membrane with 5% skim milk for 1 hour. I also wash my
gloves well before working so there is no powder. When I do a Western blot
instead of an immunoblot, I do not have this problem. If anyone has any
suggestions or advice, please write to:
ababwah at

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