Tris buffers

hsieh1 at jeflin.tju.edu hsieh1 at jeflin.tju.edu
Mon Sep 2 19:01:03 EST 1996


Hi everyone:

How about PBS?  Are there any components of powder-made PBS that would
also "gum up" antigen processing?

TIA,
Michael Hsieh
grad student

In article <199608262320.SAA24687 at wustl.edu>, brett at BORCIM.WUSTL.EDU
(brett) wrote:

> >Hi, out there...
> >
> >I remember hearing once that in mouse spleen cell proliferation assays, the
> >stimulating antigens should not be dissolved in Tris buffers.  Can anyone
> >elaborate: is this true?  Is there something about Tris buffers that is
> >incompatible with lymphocyte proliferation assays?  If so, can anyone
> >explain what it is?
> >
> >Thanks for any advice!
> >
> >Larry
> >
> >Lawrence S. D. Anthony, Ph.D.
> >Senior Scientist/Immunology
> >StressGen Biotechnologies Corp.
> >120-4243 Glanford Avenue,
> >Victoria, B.C.  V8Z 4B9
> >Tel: 604-744-2811
> >Fax: 604-744-2877
> >Web Page: http://www.stressgen.com/stressgen
> 
> I wouldn't touch any cell with a trizma buffered solution. It has a propensity
> to buffer the endocytic pathway and no doubt gums up antigen processing in
> such assays.



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