Hi, I have been trying to transfection T cell hybridoma cell line named
DO11.10 with the vector GL-3 (Promega) and so far, have not had any
success. If anybody have used this cell line in transtection expt,
please advise what conditions are the best to use? Thank you in
advance.
Xiangli Yang
xyang at bmg.bhs.uab.edu