hybridoma transfection

David Ucker duck at uic.edu
Tue Sep 17 23:22:15 EST 1996


In article <3239AC1A.46F at uabdpo.dpo.uab.edu> path016 at UABDPO.DPO.UAB.EDU (Arman Saparov) writes:
>From: path016 at UABDPO.DPO.UAB.EDU (Arman Saparov)
>Subject: hybridoma transfection
>Date: 13 Sep 1996 16:42:58 -0700

>Hi, I have been trying to transfection T cell hybridoma cell line named
>DO11.10 with the vector GL-3 (Promega) and so far, have not had any
>success.  If anybody have used this cell line in transtection expt,
>please advise what conditions are the best to use?  Thank you in
>advance.
>Xiangli Yang
>xyang at bmg.bhs.uab.edu

Electroporate:  10^7-10^8 cells in PBS,  .4cm gap, .25kV, 960uF using Biorad 
Gene Pulser
--
Kevin J. Harvey
University of Illinois at Chicago
E-Mail: micio at uic.edu



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