disulfide bonds

joachim goedhart Joachim=Goedhart%Laser%BC.WAU at VINES2.WAU.NL
Mon Apr 7 06:13:29 EST 1997


Hello,

I'm looking for a method to seperate the L and H chains of an IgG molecule. These chains are linked by 
disulfide bonds. DTT and mercaptaethanol probably destroy all disulfide bonds?!
Does anybody know a method to disrupt only the INTERmolecular disulfide bonds without destroying the 
INTRAmolecular bridges?

Thanx,

Joachim.



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