disulfide bonds

Keld Sorensen KeldS at uic.edu
Mon Apr 7 05:24:07 EST 1997


DTT and beta-mercapto will cleave ALL SS bonds.
2-MEA (mercaptyethylamine) can often be used to selectively cleave
the SS bonds in the "hinge" region, levaing the L and the H chains
attached to each other. I have not heard of anybody doing what you
describe - what would be purpose be? You would have an Ab that
does not recognize its Ag.



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