ELISA question

Jlk1117440 jlk1117440 at aol.com
Mon Apr 14 12:31:02 EST 1997

I have performed an ELISA to detect patients' reactivity with a naturally
occuring protein (about 28 kDa).  Now I have optical density readings of
the plate, but am not sure if the numbers are significant.  For example,
if one patient's reading is .250 and another's is .050, is the former
positive and the latter negative?  I would appreciate any help.

Jim Knoetgen, MD
St. Luke's - Roosevelt Hospital Center
New York, New York

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