neutrophils and PERCOLL

at at
Fri Apr 18 11:10:47 EST 1997


thanks to everyone who helped me with my last problem, but now I have an
other one:
How can I seperate neutrophils from PBMC's by the Percoll-Technique
without "loosing" them? Everytime I tried it, the cells clumped and I
was unable to redisolve them. Where can I get a proper method for
seperating neutrophils with Percoll?
Thank you for any help, Christian

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