neutrophils and PERCOLL

Jean-Philippe GÉRARD jpg at
Mon Apr 21 02:40:43 EST 1997 at ecrivait (wrote):

> How can I seperate neutrophils from PBMC's by the Percoll-Technique
> without "loosing" them? Everytime I tried it, the cells clumped and I
> was unable to redisolve them. Where can I get a proper method for
> seperating neutrophils with Percoll?

Did you try to dilute Percoll in a medium without calcium ?
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