SV40 based vector transfection in T lymphocytes

John Ladasky ladasky at leland.Stanford.EDU
Mon Apr 21 18:33:06 EST 1997


In article <ghermans-1604971810110001 at pstn10.luc.ac.be>,
Guy Hermans <hellings at luc.ac.be> wrote:
>Is it possible to transfect T lymphocytes with a vector with a SV40 replicon?
>Wil it be stable enough?

	It has been done with the Jurkat T lymphoma cell line.  Jurkat was
first transfected with a plasmid expressing the SV40 large T antigen, then
a second plasmid carrying an SV40 origin of replication and a gene of inter-
est was added.  Look for papers by Gerald R. Crabtree's group here at Stan-
ford University.

-- 
Unique ID : Ladasky, John Joseph Jr.
Title     : BA Biochemistry, U.C. Berkeley, 1989  (Ph.D. perhaps 1998???)
Location  : Stanford University, Dept. of Structural Biology
Keywords  : immunology, music, running, Green



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