Expression Cloning strategies for finding microbial CD8 antigens

David Koelle viralimm at u.washington.edu
Thu Apr 24 00:26:08 EST 1997


Any words of wisdom or literature references?  I am familiar with the
trail that led to MAGE antigens.  Anything new with making libraries in
mammalian expression vectors, vaccinia, retroviruses, etc. to perhaps sort
out CD8 antigens in a moderately large virus?  Maybe some people have
tried and failed or succeeded witht he published approaches.  I am more
interested in a direct approach than peptide pool sequencing or predicting
from motifs when the HLA restricting allele is known.

David Koelle MD
Acting Asst. Professor
Division of Allergy and Infectious Diseases
Department of Medicine, University of Washington





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