pHOOK vectors transfection, lymphocytes

Guy Hermans ghermans at luc.ac.be
Mon Apr 28 07:40:56 EST 1997


I'm considering of using the pHOOK vectors of invitrogen for transfection
experiments with T lymphocytes. Transfectants containing the pHOOK vectors
can be seperated by using magnetic beads and a magnete. 

My questions:

1. Does anyone have experience with this technique??

2. What cellines have you used for this technique??

If someone has any tips please reply

Thanks a lot

please reply to : hellings at luc.ac.be

-- 
Niels Hellings, PhD student
  Ms research Unit              Immunology research group
  Dr. L. Willems-Institute      Dept. of Physiology, LUC
  University Campus             University Campus
  B-3590 Diepenbeek             B-3590 Diepenbeek
  Belgium                       Belgium
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