cytokine intracellular stain

David Peritt Peritt at mail.med.upenn.edu
Fri Aug 1 13:42:46 EST 1997


Has anyone received good human cytokine intracellular staining for IL-5 or
IL-13.  I have a nice IFNg signal but these others are nil.

I have primed in IL4 and IL12 for 4-8 days
I stimulate with PMA/ionomycin for 4-6 hours in brefeldin A
I am gating on CD4 cells
I am losing my mind......

-- 
David Peritt
Wistar Institute
Philadelphia, PA  19104



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