Has anyone received good human cytokine intracellular staining for IL-5 or IL-13. I have a nice IFNg signal but these others are nil. I have primed in IL4 and IL12 for 4-8 days I stimulate with PMA/ionomycin for 4-6 hours in brefeldin A I am gating on CD4 cells I am losing my mind...... -- David Peritt Wistar Institute Philadelphia, PA 19104