ELISA sensitivity

Andy Fell ahfell at netmatters.co.uk
Tue Aug 5 17:22:35 EST 1997


I think you will find that if they claim "pg/ml" accuracy, they usually
mean about 800pg/ml...
Basically you have to do some careful optimisation, as there are a lot of
parameters that effect the assay, from the batch of plates to the detection
antibodies.
Andy Fell

"David L. Haviland, Ph.D." <dhavilan at IMM2.IMM.UTH.TMC.EDU> wrote in article
<3.0.32.19970729095347.0072c5ac at imm2.imm.uth.tmc.edu>...
> I do hope that discussion of this is public as this has been a topic of
> interest for me.  I have seen "kits" from Amersham, Endogen and the like
> claiming sensitivity down to the pg/ml.  I'm curious as to how this
> sensitivity is achieved.  Careful selection of capture and dectection
> antibodies?  Operationally,  with the various ELISA's and RIA's that I
have
> had to develop for various reasons, I have never been able to get the
> sensitivity better than the single digit ng/ml.  
> 
> David
> 
> 



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