Thymocyte viability

Richard Near rocket1 at bu.edu
Sun Aug 10 01:03:06 EST 1997


I've been trying to work with thymocytes in vitro.  I would just
like to be able to culture them for 24-48 hours without losing
too much viability as I am trying to induce apoptosis.  However,
every time I culture thymocytes they rapidly die (about 50% just
overnite).  Can anyone offer some tips on how to keep the thymocyte
viability at a reasonable level (at least better than 75%) after
a 24 hr + culture period. They are now just in DME 10%FCS 10%CO2
2ME 5E-5M.

Thanks
Rick
Boston U
rocket1 at bu.edu




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