Plasma and Elisa

David Verhoeven ez052183 at mailbox.ucdavis.edu
Wed Aug 13 19:22:28 EST 1997


Has anyone else had trouble getting good results from ELISAs using newborn
plasma samples?  I have been getting very high titers even on samples that
should not have any detectable titers.  I have been using 3%NFDM in TBS
and Triton X-100 as the blocking agent.  The only thing I can think of is
that this is a blocking problem.  If you have any other suggestion as to
what might be causing this, please let me know.

Thanks for the help
 
D. Verhoeven
deverhoven at ucdavis.edu




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