CD 4 cell isolation

[Robert Owe-Young]

Does anyone have a favorite method for the isolation of large numbers of
CD4 cells. We need to isolate CD4+ cells from apheresis specimens, and
our current method has gone out of production. 
We routinely do a Ficoll step first
I would like to hear from anyone who has experience with MACS, R&D, 
or Baxter systems or any other similar commercially available system.

thanks

Dr Janet Mcpherson
JJR Labs
Sydney Australia

Please reply to macpherson at angis.su.oz.au