mantle cell lymphoma bcl1

Hampton ghampton at netcom.ca
Sat Aug 23 05:50:12 EST 1997


I am trying to standardize a PCR method for Bcl1 rearrangement for mantle 
cell lymphoma. I am using primers used by several groups and published 
but am unable to reproduce results. I have done the usual Mg++ 
concentration titration and different annealing temperatures. 

Is there anyone who has had to standardize this particular rearrangement 
technique who can give me some hints or is willing to dialogue with me. I 
need help!



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