HELP: Cell lines express it all!!!!
Boolay at molbiol.uct.ac.za
Wed Aug 27 03:02:30 EST 1997
I have immortalised olfactory cell lines from embryonic mice. As part of
the characterisation, I intend to use ICC with various antibodies. I will
be using Nestin and NF160 for neuronal markers; GFAP and O1 for
non-neuronal markers and Keratin. I have used primary cultures from whole
brains to optimise the various antibodies. However, when I used the same
conditions for each antibody on the cell lines, all the cell lines
express all the antibodies. From these results, the cell lines express
both neuronal and non-neuronal markers.
I have tried optimising the individual antibodies on the cell lines - but
I`m not sure whether this is a good idea as I don`t know what the
expression pattern of each cell line is.
Can anyone advise me on how to optimise these antibodies for the
olfactory cell lines.
A reply will be greatly appreciated.
Sihaam Boolay (PhD student)
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