Fixative for IHC

A mad scientist eef1000 at cus.cam.ac.uk
Fri Aug 29 06:13:10 EST 1997


I have bene using 4% paraformaldehyde, to fix my cells, which are live
stained for Galactocerebreside-C and propidium iodide.  But I think the PF
is causing problems.  Most of the dead cells are washing off and I am
getting skewed restuls.  Can anyone suggest a different fixative?  Gal-C
is a lipid, so MetOH or EtOH are not ideal.

Ta muchly.

eef1000 at cus.cam.ac.uk

Emma
:-)

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