Stem Cell Isolation Protocals

mark mark.haynes at mail.tju.edu
Mon Dec 15 16:39:56 EST 1997


>   I'm looking for a stem cell isolation protocal.  Please let me know
> what protocals you are using and where I can find a copy of it (we are
> using mice).  Currently I am isolating stem cells using eqilibrium
> density centrifugation on a discontinuous Percoll (Pharmacia) gradient.
>        I would appreciate your suggestions,
>                                                Les III

Les I've got no suggestions unfortunately, but I was wondering how many 
stem cells do you get from one isolation?
markH



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