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December 17, 1997
Looking for a position-molecular immunology/tumor immunology
Name: Li Zhang
Title: Lecturer and Research Associate,
Degrees: Ph.D., M.D., B.S
Expertise: research
Field: immunology, molecular biology, microbiology
CURRICULUM VITAE
NAME: Li Zhang
CURRENT ADDRESS:
Department of Immunology,
The First Military Medical University,
GuangZhou, 510515 P.R.China
Tel: 0086-020-87799130
Fax: 0086-020-87705671
E-mail:fj5353 at fjnet.guangzhou.gd.cn
PERSONAL DATA:
Sex: Male.
Nationality: Chinese
Health: Excellent
Hard Working: creativity; cooperative with drive and self-motivation;
and dedicated to learn and develop new skills.
Hobbies: A good basketball, table tennis player and Computer Programs
LANGUAGE:
Fluently in English and Chinese with demonstrated good verbal=20
and written communication skills.
EDUCATION:
1996 SUN YAT-SEN University of Medical Sciences, GuangZhou, China.
Degree: Ph.D. Major: molecular immunology
1992 GuangDong Medical University, ZhanJiang,China.
Degree: M.D. Major: Medical Sciences.
1989 GuangZhou South Normal University, GuangZhou, China.
Degree: B.S. Major: Biology.
1979 School of Clinical Laboratory Technology, The first Military Medical
University, GuangZhou, China.
Major: Clinical Laboratory Technology.
CURRENT POSITION:
Lecturer and Research Associate(1992-present), Department of immunology, The=
First
Military Medical University, GuangZhou, China.
RESEARCH PROGRAMS:
1. Research Associate, Department of Immunology, The First Military Medical=
University,
GuangZhou, P.R.China, 1997-Present=20
Studying the project regarding the expression of MAGE-1-epitope-HAL-A1=
fusion proteins.
A DNA construct in which a cDNA fragment encoding the melanoma epitope=
MAGE-1 was
inserted between sequences encoding the leader and the HLA-A1 protein.=
Identified=20
recombinant plasmids by restriction enzyme analysis, Southern blot, PCR and=
sequence,=20
transformed E. Coli strain BL21 and tumor cell line with correct MAGE=
-1-epitope-HAL-A1
vector to express the recombinant proteins; extracted and purified these=
proteins by gel
filtration and column chromatography, tested the recombinant protein=
antigenicity by
ELISA and immunogenicity by animal experiment. Studying MAGE-1 recognition=
by
specific CTL and cancer immunotherapy in clinic protocol.
2. Research Associate, Department of Pathophysiology, SUN YAT-SEN University=
of
Medical Sciences, GuangZhou,China, 8/1994-12/96
The these study focuses on the investigation of the function and mechanism=
of genetically
modified tumor cells with antisense insulin-like growth factor-I (antisense=
IGF-I) as tumor
vaccines to stimulate and induce immune response. The approach/methods:=
transiently or
permanently transfect tumor cells by lipofectin, select and screen clone s=
by limited=20
dilution and drug select, analysis of RNA and DNA[molecular hybridization:=
Northern and
Southern], the expression of MHC antigens(class I and class II) confirmed by=
flow
cytometry FACS, determining CEA and AFP levels of human hepatocellular=
carcinoma cells
after gene transfer by radioimmunoassay(RIA), analyze LAK cell mediated=
cytotoxicity by
51-Chrome release assay. In vivo animal study, immunize mice with the tumor=
vaccines
and gene therapy with antisense IGF-I plasmid in established-tumor mice for=
the measurement=20
of animal survival.
3. Research Associate, Department of Immunology, The First Military Medical=
University,=20
GuangZhou, P.R.China, 8/1992-9/1993
The research of T cell vaccination against allotransplation reaction.=
Established the heart
transplation model at ear in mice for host-versus-graft reaction, T cell=
vaccination was made
from the spleen cells of BALB/C (H-2d) mice, which were induced by Con A or=
CD3
McAb were challenged with the allologous specific antigens [C57BL/6(H-2b)]=
spleen cells.
Analyzed that the phenotype(CD4+and CD8+) of T cell vaccination using flow=
cytometry
(FACS440). The heart survival at ear were measured by electrocardiograph.
4. Assistant Researcher, Department of Pathophysiology, GuangDong Medical=
University,
ZhanJiang, China, 9/1989-7/92
Involved in studying tumor immunology as related to the pathogenesis of=
infectious diseases=20
(EB virus related nasopharyngeal carcinoma, NPC). Extracted EBV by larger=
scale culture
of B95-8 and Raji cells; EBV induce the transformation of PBMC; studying the=
growth of
CNE-2Z and RAJI cells in presence of cytokines (rhIFN and rhIL-2). Using the=
methods=20
of MTT, ELISA for the measurement of cytokines (including TNF, IFN and IL-2)=
from NPC
patients.
5.Assistant Researcher, Department of Immunology, The First Military Medical=
University,
GuangZhou, P.R.China, 8/1980-9/88
* Highly skilled in all aspects of microbiology(include: culture,=
inoculation and biochemistry=20
analysis of bacterium and virus), with special emphasis on immunology=
(include: general
technology about cellular and humoral immunology, cell culture, ELISA,
immunolfluorescence, monoclonal antibody, immunocytochemistry =20
* Extensively engaged in the research of tumor immunology, set up the tumor=
metastasis
model of animal, extract the surface antigen of tumor cell, measurement of=
tumor metastasis
tissue
* Participated in the research of antigen combining cell bring Ehrilich=
ascites carcinoma host=20
and human tumor therapy by using LAK cell and tumor-infiltrating lymphocytes=
in clinic protocol
* Studying acupuncture on the effect of spontaneous lung metastasis from=
Uterine cervix cancer
U14 in mice
PROFESSIONAL SOCIETIES
The member of Chinese for Immunology
The member of Guangdong province for Immunology
SPECIALIZATION
1.Immunology(specifically tumor Immnuology) and Microbiology
2.Molecular Biology
TEACHING EXPERIENCE
Immunology -graduate and postgraduate course from 1989 to present, The First=
Military
Medical University,
ACADEMIC AWARDS:
1. Science and Technique Advance Prize of Guangdong Province for the=
research
of EBV induced specific T cell in NPC patients in 1997
2. Science and Technique Advance Prize of PLA for the research of cytokines
in NPC patients in 1995 =20
PUBLICATIONS
1.Zhang, L. ShuNong, L.CEA and AFP secretion of human hepatoma cells
transfeted by antisense IGF-I gene.
Chinese J Clinical Oncology 1997;24(6):411
2.Zhang, L. ShuNong, L. Insulin-like growth factor-I. Foreign Medical=
Sciences-
Sciences of Physiology and Pathology and Clinical 1997;17(2):174
3.Zhang, L. ShuNong, L. Effect of melanoma vaccine transfected by Antisense
IGF-I gene on the anti-metastasis activity.
J Microbiol and Immunol(Chinese) 1997;17(5):373
4.Zhang, L. ShuNong, L. The reseach of antisense IGF-I gene against mice B16
melanoma. Immunol J(Chinese) 1997;13(4):235
5.Zhang, L. ShuNong, L. The effect of antisense IGF-I gene transfer on the
expression of MHC antigens(class I and class II) of tumor cells.
Chinese J Tumor Biotherapy 1996;3(4):260
6.Zhang, L. Wang, J. The effect of T cell vaccination against=
allotransplation reaction.
Immunol J(Chinese) 1997;13(2): 85
7.Zhang, L. Zhao, MingLun. The effect of cytokines on CNE-Z and Raji=
cells.
J Chinese Immunol 1994;10(6):336
8.Zhang, L. Zhao, MingLun. The effect of Epstein-Barr virus induced=
IL-2=A1=A2TNF and
IFN in NPC patients and IgA/VCA positive individuals.
J Chinese Immunol 1993;9(supplement):181
9.Zhang, L. Zhao, MingLun. The effect of Epstein-Barr virus induced =20
supernatants of peripheral blood mononuclear cells from NPC patients=20
and IgA/VCA positive individuals on CNE-2Z and RAJI cells.
J Chinese Immunol 1993;9(supplement):186
10. Zhang, L. Effect of salt-partition moxibustion at shenque on spontanous=
lung
metastasis from Uterine Cervix cancer U14 in mice. WFAS International
Symposium on the Trend of Research in Acupuncture, Rome, 22-24 October=
=20
1992;P191
11. Zhang, L.CM-Pachymaran on the effect of spontaneous lung metastasis from
urerine cervix cancer U14 in mice. J First Milit Med=
Univ1991;11(3):256
12. Zhang, L. The improvement of nonspecial esterase stain methods of
macrophages. J First Milit Med Univ 1988;8(4):353
13. Jiang, S., Zhang, L. and Li, W. The antigen binding cells in spleen of
tumor-bearing mouse. Cancer(Chinese) 1985;1:14-20
14. Jiang, S., Zhang, L. and Li, W. The reaction of Ehrlich ascites
carcinoma-bearing host to thymus-independent antigen.
Shanghai J Immunol. 1984;4:283-286
REFERENCES
1. Li, ShuNong, M.D., Ph.D., Professor, Director, Department of=
Pathophysiology,
SUN YAT-SEN University of Medical Sciences, GuangZhou, China. 510089
Tel: 0086-020-87778223-3158(or 2398)
2. FuNing, M.D., Professor, Director, Department of Immunology, The First=
Military
Medical University,GuangZhou, China. 510515
Tel: 0086-020-87705370-48797
3. Li, Wenjian, M.D., Professor, Department of Immunology, The First=
Military
Medical University, GuangZhou, China. 510515
Tel: 0086-020-87705370-48482
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