chicken antibodies

Dom Spinella dspinella at chugaibio.com
Wed Dec 17 14:30:34 EST 1997

> Hi,
> Can anybody give me more information(or references) about raising
> antibodies in chicken (amount of protein needed, number of boosters,
> chance of antibodies against other species, etc). I heard that the
> chance on species cross-reacting antibodies is the highest in chicken;
> is that also your experience?
> Roeland Hanemaaijer
> Gaubius lab,
> Leifden, The Netherlands

Yes its true that it's easier to get species cross-reactive antibodies
(to mammalian antigens at least) by immunizing chickens.  I guess that
to a chicken immune system, all of us mammals look alike.  A protocol
that I have used to immunize chickens comes from Johnstone & Thorpe,
"Immunochemistry in Practice"; Blackwell Scientific Publishers,
London,1982; pp 28-29.  Inject 50-200 ug of protein in Complete Freund's
Adjuvant I.M into the breast muscle (2-4 sites; 0.25 - 0.5 ml per
site).  Boost in 10 days with an additional 50 - 200 ug antigen in
Incomplete Freund's. You can test bleed from the wing vein starting 7
days later.  I my hands as I recall (its been a lot of years),
additional boosters did not increase the antibody titer by much.  Good
luck.  -- Dom Spinella

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