lymphocyte proliferation re platypus

nstewart at POSTOFFICE.NEWNHAM.UTAS.EDU.AU nstewart at POSTOFFICE.NEWNHAM.UTAS.EDU.AU
Mon Dec 22 21:39:01 EST 1997


Does anyone have any ideas about what possible mitogens and methods I might
use for lymphocyte proliferation of platypus blood. I have tried ConA, LPS,
PWM and PHA, with little or no luck. The best stimulation index I got was
3:1. The platypus has a core temperature of 32C, which may be what causes
the problems. Maximum stimulation occurs at 32C with10% CO2 after 5 days
incubation. Can anyone think of any other quick, simple tests other than
lymphocyte proliferation that I could try?

Cheers Niall Stewart





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