Does anyone have any ideas about what possible mitogens and methods I might
use for lymphocyte proliferation of platypus blood. I have tried ConA, LPS,
PWM and PHA, with little or no luck. The best stimulation index I got was
3:1. The platypus has a core temperature of 32C, which may be what causes
the problems. Maximum stimulation occurs at 32C with10% CO2 after 5 days
incubation. Can anyone think of any other quick, simple tests other than
lymphocyte proliferation that I could try?
Cheers Niall Stewart
