mouse CD4+ T cells separation

tomoaki tanaka tomo826 at PO.IIJNET.OR.JP
Sat Feb 1 23:19:36 EST 1997


Now I want to isolate CD4+ T cells from mouse spleen.
I have already tried to separate CD4+ T cells from mouse spleen, using
the negative selection of affinity column , but I didn't succeed in
taking purified  CD4+ T cells because macrophages were contained.
Would you mind showing me a suitable protocol ?
Please response to my E-mail address .






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