mouse CD 4+ T cell purification

tomoaki tanaka tomo826 at PO.IIJNET.OR.JP
Sat Feb 1 22:59:47 EST 1997


Now I want to isolate CD4+ T cells from mouse spleen.
I have already tried to separate CD4+ T cells from mouse spleen, using 
the negative selection of affinity column ,but I didn't succeed in 
taking purified CD4+ T cells because macrophages were contained.
Would you mind showing me a suitable protocol?
Please response to my E-mail address.



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