mouse CD 4+ T cell purification

Kevin Ens biotex at
Mon Feb 3 09:54:00 EST 1997

tomoaki tanaka wrote:
> Now I want to isolate CD4+ T cells from mouse spleen.
> I have already tried to separate CD4+ T cells from mouse spleen, using
> the negative selection of affinity column ,but I didn't succeed in
> taking purified CD4+ T cells because macrophages were contained.
> Would you mind showing me a suitable protocol?
> Please response to my E-mail address.

You may wish to see the web site at
for more information on separating CD4+ T cells.

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