CD4+ T cell separation

Robert Jensen rjjensen at inav.net
Mon Feb 3 20:55:37 EST 1997


There are many methods to enrich for or purify CD4 cells. Panning is a
great method though magenetic methods give better purity. A first step
is having Anti CD8, Anti B220, And anti Mac-1. Incubating lymph node or
spleen cells with a cocktail of all 3 then plating washed cell
suspension on an appropriate Anti Antibody pan will  greatly increase
the % of CD4 cells



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