mouse CD 4+ T cell purification
radvanyi at oci.utoronto.ca
Mon Feb 10 22:02:28 EST 1997
On Mon, 3 Feb 1997, Kevin Ens wrote:
> tomoaki tanaka wrote:
> > Now I want to isolate CD4+ T cells from mouse spleen.
> > I have already tried to separate CD4+ T cells from mouse spleen, using
> > the negative selection of affinity column ,but I didn't succeed in
> > taking purified CD4+ T cells because macrophages were contained.
> > Would you mind showing me a suitable protocol?
> > Please response to my E-mail address.
> You may wish to see the web site at http://www.connect.ab.ca/~biotex/
> for more information on separating CD4+ T cells.
Use a nylon wool column first to deplete monocyte/macrophages and B-cells
and/or use a 2-3 h plastc adherence step prior to your CD4 selection.
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