mouse CD 4+ T cell purification

[Laszlo Radvanyi]

On Mon, 3 Feb 1997, Kevin Ens wrote:

> tomoaki tanaka wrote:
> > 
> > Now I want to isolate CD4+ T cells from mouse spleen.
> > I have already tried to separate CD4+ T cells from mouse spleen, using
> > the negative selection of affinity column ,but I didn't succeed in
> > taking purified CD4+ T cells because macrophages were contained.
> > Would you mind showing me a suitable protocol?
> > Please response to my E-mail address.
> 
> You may wish to see the web site at http://www.connect.ab.ca/~biotex/
> for more information on separating CD4+ T cells.
> 
> 

Use a nylon wool column first to deplete monocyte/macrophages and B-cells 
and/or use a 2-3 h plastc adherence step prior to your CD4 selection.


Laszlo Radvanyi