immunohistochemistry: L-selectin

Robert J Jensen rjjensen at inav.net
Wed Feb 12 22:13:32 EST 1997


L-selectin is very bright on naive lymph node T cells. I too have had
trouble with Pharmingen reagents. Try re-titering starting at 1:5 or
1:10  gating on T cells.  B cells express at lower and less uniform
levels.



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