Good rabbits or bad rabbits
mdtvg at cc.newcastle.edu.au
Sun Feb 16 19:12:22 EST 1997
> I am a PhD student at the University of Queensland, and my project involves
> raising antibodies against a particular protein.
> I started off injecting two rabbits with Freunds adjuvant mixed
The only good rabbit is a dead rabbit (rock on Mr.Calicivirus!)
Seriously, I have raised antibodies to a couple of viral proteins in
rabbits. I don't know the answer your exact problem, but I'll give you the
standard method I used which worked fine.
Primary inoculation: 1:1 emulsion of 100-200ug purified protein : COMPLETE
Freund's ( inject 5 sites intradermally dorsal surface with 0.2ml per site
AND 4 sites intramuscularly rear thigh muscles with 0.5ml per site)
Booster inoculations: 1:1 emulsion of 100-200ug purified protein :
INCOMPLETE Freund's (inject 4 sites intramuscularly rear thigh muscles with
0.5ml per site)
A couple of notes: (i) booster injection 3 weeks after primary injection,
then every two weeks thereafter as necessary (ii) some bunnies did not
respond no matter what I tried, about 1 out of every 4. If no antibodies
after 2 boosters, give up and try another rabbit
Hope this helps.
Now, if only we had an Eskimo phrase book .... ah! Here's one!
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