Peroxidase vs. Phosphatase
Ken Frauwirth BioKen
frauwirt at mendel.Berkeley.EDU
Wed Feb 19 12:20:00 EST 1997
In article <E5utnE.AvB at fsa.bris.ac.uk>,
Jared Head <bijgh at zeus.bris.ac.uk> wrote:
>I'm about to buy a conjugate for doing ELISA's with, and I have the
>choice of alkaline phosphatase or horseradish peroxidase. We are set up
>to do assays with either, and they both cost the same, so I can't make up
>my mind which to buy.
>Can anybody tell me the advantantages (or disadvantages) of the two systems?
Well, one advantage of HRP is that you can use the same enzyme-linked secondary
antibody for chemiluminescent Westerns.
Ken Frauwirth (MiSTie #33025) _ _
frauwirt at mendel.berkeley.edu |_) * |/ (_ |\ |
http://www.ocf.berkeley.edu/~frauwirt/ |_) | () |\ (_ | \|
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs
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