Fellow Netters,
I have a question on how to isolate proteins using a sepharose A
column, that is conjugated to a unknown Ab. This antibody is a
monoclonal Ab, as well as being classified as an IgG1 subtype. I want
to know what you would recommend in terms of protocols, or papers that
might help me elude the answer to the problem(No Pun Intended). I have
used the protocol from Harlow et al., directly. I tried to elute with a
low pH glycine as well as MgCl2. I tried to elute the protein, however,
all I found on my coomassie gel were bands correlating to heavy chain
and light chains of the Ab.
If you have any suggestions I would greatly appreciate an email.
ALI MCBRIDE
