ELISA High backgrounds !

frederic clement fclement at allserv.rug.ac.be
Tue Feb 25 16:24:17 EST 1997


I am trying to optimize a self-made elisa that detects IgG`s in the
supernatant of a particular cell-line.  I coat with anti-IgG and develop
with GPOD.  The Elisa is not bad, but sometimes there is too much
background.  Can anyone give me some hints to reduce the background
values??? 
Several blocking buffers tryed, still having problems.
Please help !!!

	Thanks for any sugestion !!   

Please, mail me.  My address is : fclement at allserv.rug.ac.be



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